Flagella of Escherichia coli spheroplasts

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Flagella of Escherichia coli spheroplasts.

Recently, Z. Vaituzis and R. N. Doetsch (J. Bacteriol. 89:1586, 1965) described the relationship between flagellation and motility in penicillin-induced spheroplasts of Salmonella typhimurium, and they concluded that the formation of flagella was dependent upon prior cell wall synthesis. This conclusion has now been confirmed with Escherichia coli (ATCC 13070), the results found being identical...

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The in vivo activitty of the recBC nuclease was assayed by transfection of isogenic rec+ and rec-spheroplasts with bacteriophage DNA of various origin and structure. The results indicate that the recBC nuclease can limit transfection at several stages during the production of an infective center; such limitations depend primarily on whether the DNA is in, or assumes, a nuclease-sensitive struct...

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Flagella of Salmonella Typhimurium Spheroplasts.

Vaituzis, Z. (University of Maryland, College Park), and R. N. Doetsch. Flagella of Salmonella typhimurium spheroplasts. J. Bacteriol. 89:1586-1593. 1965.-The flagella of penicillin-induced spheroplasts of Salmonella typhimurium were examined by electron and light microscopy. The process of spheroplast formation was followed for a period of 20 hr from its inception. Flagella were found to be co...

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Secretion of alkaline phosphatase subunits by spheroplasts of Escherichia coli.

Under conditions that permitted continued protein synthesis, spheroplasts of Escherichia coli were unable to form active alkaline phosphatase, although they synthesized protein that was antigenically related to alkaline phosphatase subunits. This cross-reacting protein was primarily detected in the medium of the spheroplast culture, and it had properties that closely resembled those of the alka...

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Synthetic capabilities of plasmolyzed cells and spheroplasts of Escherichia coli.

Effects of plasmolysis and spheroplast formation on deoxyribonucleic acid (DNA), ribonucleic acid (RNA), protein, and phospholipid synthesis by Escherichia coli strain THU were studied. RNA and protein synthesis were severely diminished. DNA and phospholipid synthesis were inhibited, but less so; they could be partly restored. DNA synthesis could be restored by replacing thymine in the medium w...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1966

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.91.5.2103-2104.1966